The document reading room
COA Checklist and Lot-Matching Practice
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Certificate reading checklist
Compare the lot on the container label with the lot on the certificate. Mark the report fields you find and save a text copy of your review.
This review records lot text and document fields. Verifying a certificate and linking it to a received container require supporting records.
Read the reports and compare the labels
Fictional teaching reports. All issuers, identifiers, results and criteria below are simulated.
Certificate of analysis · TCOA-024
| Field | Fictional entry |
|---|---|
| Report and authorization | TCOA-024 / Rev 01 / page 1 of 1. Issued by Training Lab 04, authorized by teaching reviewer TR-04. |
| Material | Linear hexapeptide GAGKLV with free termini. Catalog EDU-HX06. Nominal 5.0 mg peptide equivalent per vial. |
| Tested sample | One sealed vial submitted by Classroom Supplier 02. Lab accession EDULAB-0251 is mapped to supplier lot EDU-L-0042. Individual vial ID is unreported. |
| Dates | Received 2026-08-10, tested 2026-08-12, issued 2026-08-13. |
| Supporting records | Referenced LC–MS spectrum TCOA-024-M, integration TCOA-024-H, and assay calculation TCOA-024-A. These attachments are outside the exercise. |
| Analyte or attribute | Method and basis | Unit | Result | Acceptance criterion |
|---|---|---|---|---|
| GAGKLV mass match | LC–MS M-07 Rev 02, deconvoluted neutral monoisotopic mass | Da | 543.339 | 543.338 ± 0.010 Da |
| Main chromatographic peak | RP-HPLC/UV H-04 Rev 03 at 214 nm, 984 of 1,000 integrated signal-area units | area % | 98.4 | ≥97.0 area % |
| GAGKLV amount in the tested vial | Calibrated HPLC/UV assay A-02 Rev 01, assigned-content reference | mg peptide equivalent per vial | 4.70 | 4.50–5.50 mg per vial |
| Endotoxin | Method unreported | Unreported | Not reported | Unreported |
The mass differs from the stated target by +0.001 Da. The area percentage uses the integrated UV signal as its denominator. The reported amount comes from a separate calibrated assay. Ask the issuer about the scope of any unreported test.
Second report · TCOA-027
Training Lab 04 issued TCOA-027 / Rev 02 / page 1 of 1 on 2026-08-20 after testing on 2026-08-19. It lists material EDU-HX06 and lab accession EDULAB-0257. The supplier lot and the intake record that would link it to the accession are unreported.
| Analyte or attribute | Method and basis | Unit | Result | Acceptance criterion |
|---|---|---|---|---|
| GAGKLV mass match | LC–MS M-07 Rev 02, deconvoluted neutral monoisotopic mass | Da | 543.340 | 543.338 ± 0.010 Da |
| Vial label | Label lot | Report / accession | Reported lot | Document finding |
|---|---|---|---|---|
| A · BOX2-05 | EDU-L-0042 | TCOA-024 Rev 01 / EDULAB-0251 | EDU-L-0042 | Exact lot match. The individual vial link remains open. |
| B · BOX2-06 | EDU-L-0044 | TCOA-027 Rev 02 / EDULAB-0257 | Unreported | Missing link. Ask for the accession-to-lot record. |
| C · BOX2-07 | EDU-L-0043 | TCOA-024 Rev 01 / EDULAB-0251 | EDU-L-0042 | Lot conflict. Request the correct report or a documented correction. |